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  • microbiology

    hi everyone,

    i really im desperate for an answer here


    does

    TB test positive against arylsulfatase test becsuse iv been searchin and it says
    no with slow growing nd yes with rapid growing mycobateria

    2.is mycobacteria tuberculosis suppose to grow on middlebrook agar


    i thoght i post this uestion here since i ve found this forum quiet helpful.

    thanks

  • #2
    Re: microbiology

    Things have moved on since Heaf test then.
    johnep

    Comment


    • #3
      Re: microbiology

      I was gonna say. I worked in science for 10 years and havent a clue what Middlebrook agar is. Ama, worry not, I'm off out tonite but I'll look it up tomorrow if noone can give you an answer in the next few hours.
      MUR

      Major Underestimation of Resources

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      • #4
        Re: microbiology

        Don't forget a number of mycobacteria can cause TB (main one being M.Tuberculosis) and so the test you're describing is for those organisms that produce the enzyme arylsulfatase, giving a positive reaction. I don't think M.Tuberculosis gives a positive reaction but its best to check specialist microbiology texts to be sure.
        http://i620.photobucket.com/albums/t...snroses2-1.jpg

        ”We are real. We are not glam sh*t or anything else. We are Guns N’ Roses.”

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        • #5
          Re: microbiology

          arylsulfatase-so if your broth turns cloudy that means m.tuberculosis is abscent then wot have i detected then .(is it fast growing organisms such as m.fortium)

          sorry its middlebrox Thio agar plate
          does that make more sense ?


          do testing antibiotics sensitivities help when diagnosing patient with pulmonary tuberculosis?

          Comment


          • #6
            Re: microbiology

            Here are a couple of sources you might like to look at - hope it helps
            If not check out articles on PubMed

            http://www.pubmedcentral.nih.gov/pic...5&blobtype=pdf

            http://jb.asm.org/cgi/reprint/75/3/367.pdf
            http://i620.photobucket.com/albums/t...snroses2-1.jpg

            ”We are real. We are not glam sh*t or anything else. We are Guns N’ Roses.”

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            • #7
              Re: microbiology

              so if nicin positive nd nitrte positive but -rylsulftse negtive does thst men no tb nd is it only cused by m.tuberculosis or there r other bcteri

              exuse my keybord pls

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              • #8
                Re: microbiology

                Yep there are other mycobacteria that cause TB main one being M.Bovis, although all other species are rare.

                Sorry can't provide a definitive answer on the arylsulfatase questions, microbiology is not my forte
                http://i620.photobucket.com/albums/t...snroses2-1.jpg

                ”We are real. We are not glam sh*t or anything else. We are Guns N’ Roses.”

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                • #9
                  Re: microbiology

                  thanks

                  so bacterai causing pulmonary tb would be different from ones causing just TB

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                  • #10
                    Re: microbiology

                    is m.tuberculosis a fastidous or nonfastidiuos microorganism or non

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                    • #11
                      Re: microbiology

                      M.Tuberculosis is the cause of about 98% of TB cases, M.Bovis is the cause of TB in cattle (bovine TB)
                      What do you mean by fastidious ?
                      http://i620.photobucket.com/albums/t...snroses2-1.jpg

                      ”We are real. We are not glam sh*t or anything else. We are Guns N’ Roses.”

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                      • #12
                        Re: microbiology

                        i dont know im asking, becuse i want to know why no growth was on my TSA (tryptic soya gar)agar plate where there should be as doctor has diagnosed them with TB.
                        im not sure if m.tuberculosis is suppose to grow on it or not

                        Comment


                        • #13
                          Re: microbiology

                          Originally posted by Ama2007 View Post
                          is m.tuberculosis a fastidous or nonfastidiuos microorganism or non
                          MBI200 Microbiology, Fall 2004/2005 - OpenCourseWare - Tufts University
                          47 BC : Julius Cesar : Veni Vidi Vici : I came, I saw I conquered.
                          2018 AD : Modern Man : I shopped, I clicked, I collected.
                          How times change.

                          If you find you have read something that has upset or offended you an anyway please unread it at once.

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                          • #14
                            Re: microbiology

                            thanks alot that cleared things up,,

                            so how can u then explain lack of growth on TSA does that mean there was no mycobateria ,i mean should m.tuberculosis grow on TSA

                            this question is really bugging me i ve been searching evereywhere .

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