Hi

Got exams coming up guys and was wondering whether you guys could shed some light on something I'm finding hard to get my head around.

Its regarding pre-formulation studies.

When a new drug has been discovered and the pre-formulation team has been given a small sample of the drug it is important to establish two parameters:

1 - Intrinsic solubility studies
2 - pKa

Before that UV is adopted to establish as a "simple analytical technique so that all future tests are quantitative".

What I can't understand is how is UV used for solubility and pKa studies at the beginning of pre-formulation???

Also for an impure drug sample, one would use a phase-solubility method to establish the intrinsic solubility of the drug. How can this further be used then to determine pKa???

Thanks in advance.